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PUBLICATIONS

FULL LIST OF PUBLICATIONS

As independent PI

 

Selected Articles

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  1. Dell M, Tran MA, Capper MJ, Sundaram S, Fiedler J, Köhnke J*, Hellmich UA*, Hertweck C* (2023): Trapping of a Polyketide Synthase Module After C-C Bond Formation Reveals Transient Acyl Carrier Domain Interactions. Angew Chem Int Ed Engl. 10.1002/anie.202315850

  2. Adam S, Zheng D, Klein A, Volz C, Mullen W, Shirran SL, Smith BO, Kalinina OV, Müller R, Köhnke J (2023): Unusual peptide-binding proteins guide pyrroloindoline alkaloid formation in crocagin biosynthesis. Nat Chem 15(4), pp. 560-8.

  3. Sikandar A, Lopatniuk M, Lushetskyy A, Müller R, Köhnke J (2022): Total In Vitro Biosynthesis of the Thioamitide Thioholgamide and Investigation of the Pathway. J Am Chem Soc. 144, 11, 5136-5144.

  4. Sikandar A, Franz L, Adam S, Santos-Aberturas J, Horbal L, Luzhetskyy A, Truman AW, Kalinina OV, Köhnke J (2020): The bottromycin epimerase BotH defines a group of atypical α/β-hydrolase-fold enzymes. Nat Chem Biol 16(9), pp. 1013-8.

  5. Sikandar A, Cirnski K, Testolin G, Volz C, Brönstrup M, Kalinina OV, Müller R, Köhnke J (2018): Adaptation of a Bacterial Multidrug Resistance System Revealed by the Structure and Function of AlbA. J Am Chem Soc. 140(48), pp. 16641-49.

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Additional Original Articles

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  1. McGuire K, He S, Gracie J, Bryson C, Zheng D, Clark AW, Köhnke J, France DJ, Nau WM, Lee T-C, Peveler WJ (2023): Supramolecular Click Chemistry for Surface Modification of Quantum Dots Mediated by Cucurbit [7] uril. ACS Nano, 17(21), 21585-94.

  2. Sikandar A, Popoff A, Jumde RP, Mándi A, Kaur A, Elgaher WAM, Rosenberger L, Hüttel S, Jansen R, Hunter M, Köhnke J, Hirsch AKH, Kurtán T, Müller R (2023): Revision of the absolute configurations of chelocardin and amidochelocardin. Angew Chem Int Ed Engl., 62(40), e20230643.

  3. Hamid R, Adam S, Lacour A, Monjas L, Köhnke J, Hirsch AKH (2023): 1-deoxy-D-xylulose-5-phosphate synthase from Pseudomonas aeruginosa and Klebsiella pneumoniae reveals conformational changes upon cofactor binding. J Biol Chem 299(9), 105152.

  4. Kolling D, Haupenthal J, Hirsch AKH, Köhnke J (2023): Facile Production of the Pseudomonas aeruginosa Virulence Factor LasB in Escherichia coli for Structure-Based Drug Design. ChemBioChem, 24, 202300185.

  5. She S, Bell NL, Zheng D, Mathieson JS, Castro MD, Long D-L, Köhnke J, Cronin L (2023): Robotic synthesis of peptides containing metal-oxide-based amino acids. Chem 8(10), 2734-48.

  6. Testolin G, Richter J, Ritter A, Prochnow H, Köhnke J, Brönstrup M (2022): Optical Modulation of Antibiotic Resistance by Photoswitchable Cystobactamids. Chem Eur J, 28, e202201297.

  7. Kaya C, Walter I, Yahiaoui S, Sikandar A, Alhayek A, Konstantinović J, Kany AM, Haupenthal J, Köhnke J, Hartmann RW, Hirsch AKH (2021): Substrate-Inspired Fragment Merging and Growing Affords Efficacious LasB Inhibitors. Angew Chem Int Ed Engl., doi 10.1002/anie.202112295.

  8. Franz L, Köhnke J (2021): Leader peptide exchange to produce hybrid, new-to-nature ribosomal natural products. Chem Commun, 57, 6372-5.

  9. Walter I, Adam S, Gentilini MV, Kany AM, Brengel C, Thomann A, Sparwasser T, Köhnke J, Hartmann RW (2021): Structure-Activity Relationship and Mode-of-Action Studies Highlight 1-(4-Biphenylylmethyl)-1H-imidazole-Derived Small Molecules as Potent CYP121 Inhibitors. ChemMedChem

  10. Axer A, Jumde RP, Adam S, Faust A, Schafers M, Fobker M, Köhnke J+, Hirsch AKH+, Gilmour R+ (2021): Enhancing glycan stability via site-selective fluorination: modulating substrate orientation by molecular design. Chem Sci, 12, pp. 1286-94.

  11. Adam S, Franz L, Milhim M, Bernhardt R, Kalinina OV, Köhnke J (2020): Characterization of the Stereoselective P450 Enzyme BotCYP Enables the In Vitro Biosynthesis of the Bottromycin Core. J Am Chem Soc. 142, 49, pp. 20560-5.

  12. Sikandar A, Lopatniuk M, Luzhetskyy A, Köhnke J (2020): The Non-Heme Monooxygenase ThoJ Catalyzes Thioholgamide β-Hydroxylation. ACS Chem Biol, 15(10), pp. 2815-9.

  13. Hug JJ, Dastbaz J, Adam S, Revermann O, Köhnke J, Krug D, Müller R (2020): Biosynthesis of cittilins, unusual ribosomally synthesized and post-translationally modified peptides from Myxococcus xanthus. ACS Chem Biol, 15(8), pp. 2221-31.

  14. Konstantinovic J, Yahiaoui S, Alhayek A, Haupenthal J, Schönauer E, Andreas A, Kany AM, Mueller R, Köhnke J, Berger F, Bischoff M, Hartmann RW, Brandstetter H, Hirsch AKH (2020): N-Aryl-3-mercaptosuccinimides as Antivirulence Agents Targeting Pseudomonas aeruginosa Elastase and Clostridium Collagenases. J Med Chem, 63(15), pp. 8359-68.

  15. Keller L, Canuto KM, Liu C, Suzuki B M, Almatili J, Sikandar A, Naman CB, Glukhov E, Luo D, Duggan BM, Luesch H, Köhnke J, O’Donoghue AJ, Gerwick WH (2020): Tutuilamides A–C: Vinyl-Chloride-Containing Cyclodepsipeptides from Marine Cyanobacteria with Potent Elastase Inhibitory Properties. ACS Chem Biol, 15(3), pp. 751-57.

  16. Sikandar A, Franz L, Melse O, Antes I, Köhnke J (2019): Thiazoline-Specific Amidohydrolase PurAH Is the Gatekeeper of Bottromycin Biosynthesis. J Am Chem Soc. 141(25), pp. 9748-52.

  17. Adam S, Klein A, Surup F, Köhnke J (2019): The structure of CgnJ, a domain of unknown function protein from the crocagin gene cluster. Acta Cryst. F 75(3), pp. 205-11.

  18. Kany AM, Sikandar A, Yahiaoui S, Haupenthal J, Walter I, Empting M, Köhnke J, Hartmann RW (2018): Tackling Pseudomonas aeruginosa Virulence by a Hydroxamic Acid-Based LasB Inhibitor. ACS Chem Biol 13(9), pp. 2449-55.

  19. Kany AM, Sikandar A, Haupenthal J, Yahiaoui S, Maurer C, Proschak E, Köhnke J, Hartmann RW (2018): Binding Mode Characterization and Early in vivo Evaluation of Fragment-like Thiols as Inhibitors of the Virulence Factor LasB from Pseudomonas aeruginosa. ACS Infect Dis., 4(6), pp. 988-97.

  20. Franz L, Adam S, Santos-Aberturas J, Truman AW, Köhnke J (2017): Macroamidine formation in bottromycins is catalyzed by a divergent YcaO enzyme. J Am Chem Soc. 139(50), pp. 18158-61.

  21. Fu C, Sikandar A, Donner J, Zaburannyi N, Herrmann J, Reck M, Wagner-Döbler I+, Köhnke J+, Müller R+ (2017): The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD. Nat Comm, 8(1): 1529.

  22. Kjaerulff L, Sikandar A, Zaburannyi N, Adam S, Herrmann J, Köhnke J, Müller R (2017) Thioholgamides: Thioamide-Containing Cytotoxic RiPP Natural Products. ACS Chem Biol, 12(11), pp. 2837-41.

  23. Beshr G, Sikandar A, Jemiller EM, Klymiuk N, Hauck D, Wagner S, Wolf E, Köhnke J+, Titz A+ (2017) Photorhabdus luminescens lectin A (PllA)-a new probe for detecting α-galactoside-terminating glycoconjugates. J Biol Chem, 292(48), pp. 19935-51.

  24. Viehrig K, Surup F, Volz C, Herrmann J, Abou Fayad A, Adam S, Köhnke J, Trauner D, Müller R (2017): Structure and Biosynthesis of Crocagins: Polycyclic Postranslationally Modified Ribosomal Peptides from Chondromyces crocatus. Angew Chem Int Ed Engl., 56(26), pp. 7407-10.

  25. Mann G, Huo L, Adam S, Nardone B, Vendome J, Westwood NJ, Müller R, Köhnke J (2016): Structure and substrate recognition of the Bottromycin maturation enzyme BotP. Chembiochem, 17(23), pp. 2286-92.

  26. Sucipto H, Sahner JH, Prusov E, Wenzel SC, Hartmann R, Köhnke J+, Mueller R+ (2015): In vitro reconstitution of α-pyrone ring formation in myxopyronin biosynthesis. Chem Sci. 6, pp. 5076-85.

 

PostDoc

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  1. Köhnke J, Mann G, Bent AF, Ludewig H, Shirran S, Botting C, Lebl T, Houssen WE, Jaspars M, Naismith JH (2015): Structural analysis of leader-peptide binding enables leader-free cyanobactin processing. Nat Chem Biol., 11(8), pp. 558-63.

  2. Mann G, Köhnke J, Bent AF, Graham R, Houssen W, Jaspars M, Schwarz-Linek U, Naismith JH (2014): The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster. Acta Cryst. F 70(12), pp. 1597-603.

  3. Houssen WE, Bent AF, McEwan AR, Pieiller N, Tabudravu J, Köhnke J, Mann G, Adaba RI, Lebl T, Hawas UW, Liu H, Schwarz-Linek U, Smith MC, Naismith JH, Jaspars M (2014): An efficient method for the in vitro production of azol(in)e-based cyclic peptides. Angew Chem Int Ed Engl. 53(51), pp. 14171-4.

  4. Köhnke J, Bent AF, Zollman D, Smith K, Houssen WE, Zhu X, Mann G, Lebl T, Scharff R, Shirran S, Botting CH, Jaspars M, Schwarz-Linek U, Naismith JH (2013): The Cyanobactin Heterocyclase Enzyme: A Processive Adenylase That Operates with a Defined Order of Reaction. Angew Chem Int Ed Engl. 52(52), pp. 13991-6.

  5. Köhnke J, Morawitz F, Bent AF, Houssen WE, Shirran SL, Fuszard MA, Smellie IA, Botting CH Smith MC, Jaspars M, Naismith JH (2013): An Enzymatic Route to Selenazolines. Chembiochem. 14(5), pp. 564-7.

  6. Bent AF, Köhnke J, Houssen WE, Smith MCM, Jaspars M, Naismith JH (2013): Structure of PatF from Prochloron didemni. Acta Cryst. F 69(6), pp. 618-23.

  7. Köhnke J, Bent A, Houssen WE, Zollman D, Morawitz F, Shirran S, Vendome J, Nneoyiegbe AF, Trembleau L, Botting CH, Smith MC, Jaspars M, Naismith JH (2012): The mechanism of patellamide macrocyclization revealed by the characterisation of the PatG macrocyclase domain. Nat Struct Mol Biol. 19(8), pp. 767-72.

  8. Houssen WE*, Köhnke J*, Zollman D, Vendome J, Raab A, Smith MC, Naismith JH, Jaspars M (2012): The Discovery of New Cyanobactins from Cyanothece PCC 7425 Defines a New Signature for Processing of Patellamides. Chembiochem. 13(18), pp. 2683-9.

 

PhD

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  1. Köhnke J, Jin X, Trbovic N, Katsamba PS, Brasch J, Ahlsen G, Scheiffele P, Honig B, Palmer AG 3rd, Shapiro L (2008): Crystal Structures of beta-Neurexin 1 and beta-Neurexin 2 Ectodomains and Dynamics of Splice Insertion Sequence 4. Structure. 16(3), pp 410-21.

  2. Köhnke J, Katsamba PS, Ahlsen G, Bahna F, Vendome J, Honig B, Shapiro L, Jin X (2010): Splice form-dependence of b-neurexin/neuroligin binding interactions. Neuron 67(1), pp. 61-74.

  3. Browne WB, Sookraj KA, Vishnevetsky M, Adler V, Sarafraz-Yazdi E, Lou S, Könhke J, Shteyler V, Ikram K, Harding M, Bluth MH, Ng M, Brandt-Rauf PW, Hannan R, Bradu S, Zenilman ME, Michl J, Pincus MR (2008): The penetratin sequence in the anticancer PNC-28 peptide causes tumor cell necrosis rather than apoptosis of human pancreatic cancer cells. Ann Surg Oncol. 15(12), pp. 3588-600.

  4. Köhnke J, Jin X, Budreck EC, Posy S, Scheiffele P, Honig B, Shapiro L (2008): Crystal structure of the extracellular cholinesterase-like domain from neuroligin-2. Proc Natl Acad Sci U S A. 105(6), pp. 1873-8.

 

Pre PhD

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  1. Jakobs A*, Köhnke J*, Himstedt F, Funk M, Korn B, Gaestel M, Niedenthal R (2007): Ubc9 fusion-directed SUMOylation (UFDS): A method to analyze function of protein SUMOylation. Nature Methods. 4(3), pp. 245-250.

  2. Hellenbroich Y, Schulz-Schaeffer W, Nitschke MF, Köhnke J, Händler G, Bürk K, Schwinger E, Zühlke C (2004): Coincidence of a large SCA12 repeat allele with a case of Creutzfeld-Jacob disease. J Neurol Neurosurg Psychiatry 75(6), pp 937-8.

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Reviews

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  1. Franz L, Kazmaier U, Truman AW, Köhnke J (2021): Bottromycins - biosynthesis, synthesis and activity. Nat Prod Rep 38, 1659-1683

  2. Montalbán-López M, Scott TA, Ramesh S, Rahman IR, van Heel AJ, Viel JH, Bandarian V, Dittmann E, Genilloud O, Goto Y, Grande Burgos MJ, Hill C, Kim S, Köhnke J, Latham JA, Link AJ, Martínez B, Nair SK, Nicolet Y, Rebuffat S, Sahl HG, Sareen D, Schmidt EW, Schmitt L, Severinov K, Süssmuth RD, Truman AW, Wang H, Weng JK, van Wezel GP, Zhang Q, Zhong J, Piel J, Mitchell DA, Kuipers OP, van der Donk WA (2020): New developments in RiPP discovery, enzymology and engineering. Nat Prod Rep 38 (1), 130-239.

  3. Sikandar A, Köhnke J (2019): The role of protein–protein interactions in the biosynthesis of ribosomally synthesized and post-translationally modified peptides. Nat Prod Rep 36(11), pp. 1576-88.

  4. Köhnke J, Bent AF, Houssen W, Mann G, Jaspars M, Naismith JH (2014): The structural biology of patellamide biosynthesis. Curr Opin Struct Biol 29, pp. 112-21.

 

Patents (5)

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Pending

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US Patent App. 15/524,219

Modified Heterocyclase

 

US Patent App. 15/683,266

In vitro production of cyclic peptides

 

EU Application No. 17210536.3-1120

Gene cluster for the biosynthetic production of tetracycline compounds in a heterologous host

 

 

Issued

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Patent No.

A method for determining SUMOylation.

 

Patent No. 04701742.1-2405-US2004000684

Peptides selectively lethal to malignant and transformed mammalian cells.

 

Books (1)

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Cyclic peptides: From Bioorganic Synthesis to Applications

Editors: Köhnke J, Naismith JH, van der Donk WA

Royal Society of Chemistry, 2017

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